LC-MS/MS vs HPLC for peptide analysis — November 2025

TrialTracker_MD said:

Vendor quality matters when discussing certificates of analysis.

This answered a question I did not know how to ask. Sending this to two other people who asked me the same thing last week.

Adding the clinical framing, because it changes how the question reads.

Third-party testing services for certificates of analysis besides Janoshik:

• Janoshik: Gold standard for peptide community, ~$156, 10-14 day turnaround
• Valitech: US-based, ISO 17025 accredited, ~$236
• Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
• ChemTox: Basic identity and purity testing, ~$126

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

DebRD_ATL said:

Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$156, 10-14 day turnaround…

Same pattern here, and in the same order. Nothing to add that would improve it.

DebRD_ATL said:

Third-party testing services for certificates of analysis besides Janoshik: Janoshik: Gold standard for peptide community, ~$156, 10-14 day turnaround…

There is a second half to this that has not been said yet. Worth answering the question that was asked rather than the one behind it. The narrow version usually has an answer; the broad version usually does not, and answering the broad one is how a thread stops being useful.

One concrete data point for the thread. Practical: whatever you change, write down the date and the reason. In three months the reason is what you will have forgotten, and the reason is what makes the record worth having.