Has anyone dealt with moisture content analysis for lyophilized peptides?

Adding the clinical framing, because it changes how the question reads.

Vendor quality matters when discussing certificates of analysis. I've tested my last 6 batches through Janoshik and purity has ranged from 98.9% to 100.7%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

Dr.RaviCardio said:

HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean: Purity %: Should be…

Bookmarking. The distinction being drawn above is the one nobody else makes.

Adding the clinical framing, because it changes how the question reads.

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:

Main peak retention time: 15.8 minutes (consistent with semaglutide reference standard)
Peak purity: 98.1%
Related substances: 0.8% (single impurity at 9.4 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.6 (matches expected molecular weight)

This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.

TrialNerd_Beth said:

My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…

Mine went the same way, slower.

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