Has anyone dealt with moisture content analysis for lyophilized peptides?

Dr.GutHealth said:

Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight.

Third-party testing services for certificates of analysis besides Janoshik:

• Janoshik: Gold standard for peptide community, ~$134, 10-14 day turnaround
• Valitech: US-based, ISO 17025 accredited, ~$244
• Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
• ChemTox: Basic identity and purity testing, ~$124

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

MikeFit_NJ said:

One addition: an unstated method makes a report close to uninterpretable.

Genuinely useful, thank you. I had the facts and not the framework. Printing the relevant bit and taking it with me.

Dr.GutHealth said:

Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight.

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

• No HPLC data: If there's no chromatography graph, it's not a real COA
• Exact 100% purity: Suspicious — real testing always shows slight variation
• No batch number: Can't trace back to specific production
• No endotoxin testing: For injectables, this is essential
• Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

zoe_NC said:

Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

I tested my certificates of analysis batch through Janoshik — 99.2% purity. That's within acceptable range for compounded peptides. Turnaround was 10 business days.

The test costs ~$122 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.

Moderator note: a couple of off-topic posts removed. Tagging this one for the weekly digest.