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Evidence-based GLP-1 & peptide discussion since 2023
ForumsCOA & Analytical TestingHow to commission independent testing — need advice Page 4

How to commission independent testing — need advice

SkepticalSean Sat, Feb 22, 2025 at 7:31 PM 16 replies 1,834 viewsPage 4 of 4
VanRx_Mike
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Feb 27, 2025 at 2:58 PM#16

From the other side of the consultation, briefly.

COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:

  • No HPLC data: If there's no chromatography graph, it's not a real COA
  • Exact 100% purity: Suspicious — real testing always shows slight variation
  • No batch number: Can't trace back to specific production
  • No endotoxin testing: For injectables, this is essential
  • Template looks like it was made in Word: Professional labs have standardized formats

I have seen fake COAs that are literally photoshopped. Always verify independently if possible.

49 22Dr.LeslieOBGYN, MikeNYC_runner and 46 others
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LindaRN_retired
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Sarasota, FL
Feb 28, 2025 at 6:24 AM#17
Dr.PeteFamMed said:
My vendor review relevant to certificates of analysis: ordered 5 times from my current compounder.

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 46 submitted tests:

  • 82% tested at ≥98% purity
  • 12% tested at 95-98% purity
  • 6% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

50 23Dr.GutHealth, amsterdam_pete, LondonLisa and 47 others
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bri_stats
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Seattle, WA
Feb 28, 2025 at 9:49 PM#18
Dr.PeteFamMed said:
My vendor review relevant to certificates of analysis: ordered 5 times from my current compounder.

Third-party testing services for certificates of analysis besides Janoshik:

  • Janoshik: Gold standard for peptide community, ~$145, 10-14 day turnaround
  • Valitech: US-based, ISO 17025 accredited, ~$225
  • Eurofins: Full pharmaceutical testing suite, more expensive but comprehensive
  • ChemTox: Basic identity and purity testing, ~$105

Janoshik is the community standard but any ISO-accredited analytical lab can verify peptide identity and purity. The key is independence — don't rely solely on the vendor's own testing.

Last edited: Mar 1, 2025 at 1:49 AM
1 24TomFromTexas
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
TrialTracker_MD
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Jan 2024
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Mar 1, 2025 at 1:14 PM#19

Following on from Dr.PeteFamMed — and this may be the naive question:

Did your prescriber agree with that reading, and if not what was their objection?

2 0Dr.PulmRoch, maya_sedona
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Admin
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Mar 2, 2025 at 4:39 AM#20

Moderator note: a couple of off-topic posts removed.

Last edited: Mar 2, 2025 at 8:39 AM
24 22PeptideSynthNJ, Dr.KarenChen, Dr.NateNeph and 21 others
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