Janoshik vs in-house testing — third-party verification importance

LibrarianMeg said:

Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight.

Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 47 submitted tests:

• 75% tested at ≥98% purity
• 18% tested at 95-98% purity
• 7% tested at <95% purity (concerning)

The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.

TrialTracker_MD said:

One addition: an unstated method makes a report close to uninterpretable.

This is exactly what I could not find anywhere else.

LibrarianMeg said:

Mass spectrometry confirmation for certificates of analysis: beyond HPLC purity, mass spec tells you the molecule's exact molecular weight.

Vendor quality matters when discussing certificates of analysis. I've tested my last 6 batches through Janoshik and purity has ranged from 97.3% to 100.7%. Consistency is key.

For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.

PurityPaulOR said:

Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.

I tested my certificates of analysis batch through Janoshik — 99.8% purity. That's within acceptable range for compounded peptides. Turnaround was 8 business days.

The test costs ~$143 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.

Moderator note: two posts asking for a source have been merged into one. Please search the thread before asking again.