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ForumsDosing & ProtocolsSteady-state concentrations across dose tiers — anyone have experience? Page 2

Steady-state concentrations across dose tiers — anyone have experience?

Dr.ReproEndo Thu, Sep 18, 2025 at 2:39 PM 47 replies 1,788 viewsPage 2 of 10
RetaRick_CA
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Sep 18, 2025 at 4:16 PM#6
DataDave said:
Do it in two steps and it stops being confusing.

I am going to disagree with reconstituting low as a general rule. More diluent, more punctures, more in-use days at room temperature, and the stability trade-off is real. Precision is not the only variable being optimised.

Last edited: Sep 18, 2025 at 5:16 PM
16 11SkepticalSean, Dr.CardioMD, EndoResFellow and 13 others
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SleepDoc_PDX
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Sep 18, 2025 at 4:54 PM#7
Dr.ReproEndo said:
I reconstituted a 10mg vial with 2ml of bacteriostatic water and I want someone to check my unit arithmetic before I draw anything, because I keep…

For anyone reconstituting dosing arithmetic peptides: the water-to-powder ratio determines your concentration. Here's my reference:

Vial SizeBAC WaterConcentration0.25mg Dose
5mg2mL2.5mg/mL10 units
5mg2.5mL2mg/mL12.5 units
10mg2mL5mg/mL5 units
10mg3mL3.33mg/mL7.5 units

More water = easier to measure small doses but vial runs out faster. Find the balance that works for your dose.

Last edited: Sep 18, 2025 at 7:54 PM
15 10hank_denver, carlos_SATX, sophie_paris and 12 others
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Dr.NutriCornell
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Ithaca, NY
Sep 18, 2025 at 5:32 PM#8
RetaRick_CA said:
I am going to disagree with reconstituting low as a general rule.

Ongoing cost tracking for dosing arithmetic — transparency for the community:

Monthly medication cost: $134
Supplies (syringes, BAC water, alcohol swabs): ~$12/month
Janoshik testing: ~$14/month (amortized quarterly)
Lab work: ~$54/month (amortized quarterly)
Total: ~$194/month

Compare to brand Wegovy at $1274/month without insurance. Compounded is roughly 85% cheaper for what I have found to be clinically equivalent medication.

14 9laura_annarbor, JenMemphis, pat_auckland and 11 others
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tommy_boulder
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Sep 18, 2025 at 6:10 PM#9

Following on from sophie_paris — and this may be the naive question:

How much material I am losing to dead space, and whether that explains why a 10mg vial gives me nine usable draws rather than ten?

13 8dan_philly, MeganSA_TX, LarryQC_SD and 10 others
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Dr.ReproEndo
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Sep 18, 2025 at 9:12 PM#10

Reporting back.

Resolved. I wrote mg, ml and mg/ml on the vial in marker and did the division in that order, and the two of us now get the same number every time.

Last edited: Sep 18, 2025 at 10:12 PM
1 1carl_compliance
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