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ForumsCompounding & FormulationHas anyone dealt with dumb question - what is bacteriostatic water exactly?

Has anyone dealt with dumb question - what is bacteriostatic water exactly?

NauseaFreeNow Thu, Apr 4, 2024 at 10:28 PM 49 replies 2,765 viewsPage 1 of 10
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NauseaFreeNow
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Apr 4, 2024 at 10:28 PM#1

Two of us with identical vials and identical diluent arrived at different unit counts, which means one of us has a conceptual error rather than a rounding error.

The three numbers to write on the vial: total mg, total ml, and mg per ml. Everything else is division. And the sanity check is that dose volume times number of doses should be less than the volume you put in, because dead space takes the difference.

The question I want answered is how to check the arithmetic without trusting a website calculator, since three calculators gave me three answers.

Practical detail welcome, however dull — the duller the better.

5 8stefan_berlin, Dr.EM_Chicago, pete_RVA and 2 others
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Dr.RenalNash
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Apr 4, 2024 at 11:22 PM#2

Short answer first, then the reasoning. Dead space is the answer to the missing dose. A fixed-needle insulin syringe holds a few microlitres in the hub and needle after the plunger bottoms out, and on small draws that is a measurable percentage of every dose. Across ten draws it adds up to most of an eleventh, which is exactly the "nine draws from a ten-dose vial" complaint. Luer-lock syringes are worse; low-dead-space fixed-needle designs are better.

6 9pam_stl, wei_SG, cory_ATX and 3 others
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ben_calgary
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Apr 5, 2024 at 12:16 AM#3
Dr.RenalNash said:
Dead space is the answer to the missing dose.

That is correct as far as it goes, and here is where it stops going. The arithmetic is right and one caveat matters: a U-40 syringe changes the markings, not the volumes. If someone hands you U-40 and you read it as U-100 you will be 2.5 times out, and that error has a direction — it is always an overdose.

7 10ZaraB_AL, JakeSmashed95, NauseaFreeNow and 4 others
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Sigma-Aldrich — Research-Grade Standards

Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.

Shop Reference Standards
anna.melb_AU
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Apr 5, 2024 at 1:10 AM#4
NauseaFreeNow said:
Two of us with identical vials and identical diluent arrived at different unit counts, which means one of us has a conceptual error rather than a…

Same position here, arrived at the long way round. Do it in two steps and it stops being confusing. First concentration: 10mg into 2ml is 5mg/ml. Then volume: a 0.5mg dose is 0.5 ÷ 5 = 0.1ml. Then units, and this is where people go wrong — a U-100 syringe is graduated in hundredths of a millilitre, so 0.1ml is 10 units. The word "units" has nothing to do with milligrams; it is a volume marking that exists because insulin happens to come at 100 units per ml.

I would rather be corrected than agreed with, if it comes to it.

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PharmD_Rodriguez
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Apr 5, 2024 at 6:17 AM#5

From the other side of the consultation, briefly.

Peptide calculator app recommendation for dosing arithmetic: I use DoseRight to calculate reconstitution volumes and injection doses. Eliminates math errors completely.

Input: vial size (mg), desired concentration (mg/mL), prescribed dose (mg)
Output: BAC water volume (mL), injection volume (units on insulin syringe)

A math error with injectable medication can mean underdosing or overdosing. Use a calculator every time until the math becomes second nature.

Last edited: Apr 5, 2024 at 11:17 AM
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