Adding the clinical framing, because it changes how the question reads. Order of operations matters more than any single choice here: establish a baseline, change one thing, wait long enough for it to express itself, then measure again under the same conditions.
The figures, for anyone assembling their own picture. The boring version of this is the one that works, and the boring version is: measure a baseline, change one variable, wait, measure again under the same conditions. Nobody wants that answer and it is still the answer.
Following on from bri_stats — and this may be the naive question:
What did you change at the same time, and can you separate the two now?
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Browse GL Biochemlisa_labSD said:The boring version of this is the one that works, and the boring version is: measure a baseline, change one variable, wait, measure again under the…
I do not accept the framing. The question has been narrowed to the version that has a tidy answer, and the part that was dropped is the part the OP actually asked about.
Happy to go further on any of that.